The root of Sophora flavescens has been reported to possess antitumor activ
ity in Sarcoma 180, lymphoid leukemia 1210 and melanotic melanoma, We have
isolated four cytotoxic flavonoids with a lavan-dulyl side-chain at C8 and
tested for their effects on human myeloid leukemia HL-60 cells and human he
patocarcinoma HepG2 cells, in terms of inhibition of proliferation and indu
ction of apoptosis, They showed potent antiproliferative effects with IC50
values from 11.3 mu M to 18.5 mu M in HL60 cells and from 13.3 mu M to 36.2
mu M in HepG2 cells, Treatment of HL-60 cells with the lavandulyflavonoids
induced apoptosis in a dose-dependent manner, Apoptosis was judged by the
detection of DNA fragmentation by agarose gel electrophoresis and the degre
e of apoptosis was quantified by a sandwich enzyme immunoassay. The hydrati
on of C4 "'C5 "' double bond with or without C3 hydroxylation caused a comp
lete loss of cytotoxicity. These results suggest that the lavanduyl side-ch
ain is essential for the activity of the flavonoids isolated from S. flaves
cens which may be used as cancer chemotherapeutic and chemopreventive agent
s. (C) 2000 Elsevier Science Ltd. All rights reserved.