Passive protection of serum from volunteers inoculated with attenuated strain 638 of Vibrio cholerae O1 in animal models

As part of the studies to obtain an oral vaccine against cholera disease, t he protective effect of serum from volunteers inoculated in a controlled tr ial with a candidate live attenuated vaccine of Vibrio cholerae O1. El Tor Ogawa (638; CTX phi mutant, hap::celA), was tested. It was confirmed that t he serum, as well as the purified IgG and IgA from the volunteers had a pro tective effect in both of the animal models used, although the purified ant ibodies needed the presence of complement to be protective. These results e mphasize the expectations about the protective potential of the candidate i n challenge studies in humans to be conducted very soon. (C) 2000 Elsevier Science Ltd. All rights reserved.