High ELISA backgrounds in virus testing of Chlorophytum comosum (Thun.) Jacques

Authors
Nicolaisen, M Nielsen, SL Scheel, C Morris, J Barker, I
Citation
M. Nicolaisen et al., High ELISA backgrounds in virus testing of Chlorophytum comosum (Thun.) Jacques, Z PFLANZENK, 107(4), 2000, pp. 376-379
Citations number
4
Categorie Soggetti
Plant Sciences
Journal title
ZEITSCHRIFT FUR PFLANZENKRANKHEITEN UND PFLANZENSCHUTZ-JOURNAL OF PLANT DISEASES AND PROTECTION
ISSN journal
03408159 → ACNP
Volume
107
Issue
4
Year of publication
2000
Pages
376 - 379
Database
ISI
SICI code
0340-8159(200007)107:4<376:HEBIVT>2.0.ZU;2-8
Abstract
High absorbance values in ELISA testing tor tospoviruses were observed in C hlorophytum comosum. On the basis of KIP-PCR, inoculation to rest plants, e lectron microscopy, and immunoblotting it was concluded that these results were caused by an unspecific reaction. This background reaction could be el iminated by addition of unspecific antiserum as a blocking agent.