Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model

Functional cartilaginous constructs for scientific research and eventual ti ssue repair were cultivated in bioreactors starting from chondrocytes immob ilized on polymeric scaffolds. The scaffolds gradually degraded as the cell s regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation con ditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Cal culated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samp les cultured at two different oxygen tensions for various periods of time.