Metabolic evidence for sequestration of low-density lipoprotein in abdominal aorta of normal rabbits

In rabbits, atherosclerosis develops preferentially at branch sites compare d with the adjacent uniform aorta. This study investigated the hypothesis t hat low-density lipoprotein (LDL) is "sequestered" (present in a form that exchanges slowly with plasma LDL) in the aortas of normal rabbits and that more LDL is sequestered at branch sites. Thus 33 normal rabbits were inject ed with LDL labeled with I-125-labeled tyramine cellobiose ( I-125-TC) to t race both undegraded LDL and aortic LDL degradation products. For 25 rabbit s, LDL was also labeled with I-131 to trace undegraded LDL alone. The time- dependent aortic I-125-TC and I-131 accumulation was determined from 0.6 to 120 h after injection. Compartmental modeling provided metabolic evidence for sequestration of LDL at the branch (P < 0.01) and uniform (P < 0.005) a bdominal aorta. Concentrations of sequestered LDL were 109 +/- 28% higher ( P < 0.0005) for branch sites. LDL mean residence time was 23.5 +/- 3.1 h fo r branch sites, 7.6 +/- 3.5 h longer (P < 0.05) than for the uniform abdomi nal aorta. Enhanced retention of higher concentrations of sequestered LDL a t branch sites could account for the increased susceptibility of these aort ic sites to atherosclerosis.