To understand molecular mechanisms of retinal development, genes expressed
selectively only in the early stage of retinal development were isolated by
subtractive hybridization based on suppression polymerase chain reaction.
The retina has no layered structure in 7-day chick embryos, in contrast wit
h the fully developed multilayered structure of neurons in 15-day embryos.
The subtraction between cDNA derived from retinal tissues at these differen
t stages, followed by repeat rounds of 5'-RACE (rapid amplification of cDNA
ends) and 3'-RACE, led to isolation of a novel gene with an open reading f
rame encoding a putative protein with 753 amino acids. Its specific express
ion in the 7-day embryonic retina was confirmed by Northern blot analysis.
The gene, named "retinovin," would be used as a marker for identifying reti
nal stem cells present at the early stage of retinal development. (C) 2000
Academic Press.