M. Hayashi et al., Hepatic copper accumulation induces DNA strand breaks in the liver cells of Long-Evans Cinnamon strain rats, BIOC BIOP R, 276(1), 2000, pp. 174-178
Citations number
27
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Effects of accumulation of copper and iron on the production of DNA strand
breaks were investigated in Long-Evans Cinnamon (LEC) strain rats that spon
taneously develop fulminant hepatitis. Copper and iron accumulated in the l
iver of LEC rats in an age-dependent manner from 4 to 15 weeks. Low-copper
food prevented the accumulation of copper in the liver, but did not prevent
accumulation of iron. When the amounts of DNA single strand breaks were es
timated by comet assay, the number of DNA strand breaks in the liver cells
of rats fed standard food increased with age from 4 to 15 weeks. The number
of DNA strand breaks in the liver cells from rats fed low-copper food were
the same as those of rats at 4 weeks of age. Thus, the copper accumulation
in the liver of LEC rats induced DNA strand breaks, but accumulation of ir
on did not. (C) 2000 Academic Press.