Hepatic copper accumulation induces DNA strand breaks in the liver cells of Long-Evans Cinnamon strain rats

Citation
M. Hayashi et al., Hepatic copper accumulation induces DNA strand breaks in the liver cells of Long-Evans Cinnamon strain rats, BIOC BIOP R, 276(1), 2000, pp. 174-178
Citations number
27
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
276
Issue
1
Year of publication
2000
Pages
174 - 178
Database
ISI
SICI code
0006-291X(20000916)276:1<174:HCAIDS>2.0.ZU;2-8
Abstract
Effects of accumulation of copper and iron on the production of DNA strand breaks were investigated in Long-Evans Cinnamon (LEC) strain rats that spon taneously develop fulminant hepatitis. Copper and iron accumulated in the l iver of LEC rats in an age-dependent manner from 4 to 15 weeks. Low-copper food prevented the accumulation of copper in the liver, but did not prevent accumulation of iron. When the amounts of DNA single strand breaks were es timated by comet assay, the number of DNA strand breaks in the liver cells of rats fed standard food increased with age from 4 to 15 weeks. The number of DNA strand breaks in the liver cells from rats fed low-copper food were the same as those of rats at 4 weeks of age. Thus, the copper accumulation in the liver of LEC rats induced DNA strand breaks, but accumulation of ir on did not. (C) 2000 Academic Press.