Evidence for the cluster model of mitochondrial steroid hydroxylase systemderived from dissociation constants of the complex between adrenodoxin reductase and adrenodoxin
T. Hara et al., Evidence for the cluster model of mitochondrial steroid hydroxylase systemderived from dissociation constants of the complex between adrenodoxin reductase and adrenodoxin, BIOC BIOP R, 276(1), 2000, pp. 210-215
Citations number
27
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Using biotinylated adrenodoxin and avidin-Sepharose 4B, dissociation consta
nts for the complex between adrenodoxin reductase and adrenodoxin in the ox
idized and reduced states were determined as 50 +/- 11 and 296 +/- 44 nM, r
espectively. Concentrations of adrenodoxin reductase/adrenodoxin in the mat
rix fraction from bovine adrenal cortex, liver, and kidney mitochondria wer
e determined to be 20.2 +/- 10.6 mu M/ 120 +/- 23 mu M, 0.17 +/- 0.06 mu M/
1.79 +/- 0.24 mu M, and 0.40 +/- 0.23 mu M/1.33 +/- 0.26 mu M, respectively
. The calculation of the percentage of adrenodoxin reductase in the complex
form in the reduced state showed that it is higher than 99% in adrenal cor
tex mitochondria, providing clear evidence for the cluster model for the mi
tochondrial steroid hydroxylase system. (C) 2000 Academic Press.