An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b

Authors
Lemercinier, X Jones, C
Citation
X. Lemercinier et C. Jones, An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b, BIOLOGICALS, 28(3), 2000, pp. 175-183
Citations number
27
Categorie Soggetti
Microbiology
Journal title
BIOLOGICALS
ISSN journal
10451056 → ACNP
Volume
28
Issue
3
Year of publication
2000
Pages
175 - 183
Database
ISI
SICI code
1045-1056(200009)28:3<175:ANSITF>2.0.ZU;2-9
Abstract
We describe the use of Nuclear Magnetic Resonance (NMR) spectroscopy to con trol the identity of purified bulk capsular polysaccharide [called poly(rib osylribitolphosphate) or PRP] from Haemophilus influenzae type b (Hib), and derivatised forms, used in the production of Hib polysaccharide-protein co njugate vaccines. We describe the approaches we have developed to validate this teal. (C) 2000 The International Association for Biologicals.