Comparison of the inactivation of canine and bovine parvovirus by freeze-drying and dry-heat treatment in two high purity factor VIII concentrates

The inactivation of bovine parvovirus (BPV) and canine parvovirus (CPV) by freeze-drying and terminal dry-heal treatment at 80 degrees C for 72 h has been investigated in two high purity factor VIII concentrates. In one produ ct, CPV was slightly more resistant to freeze-drying compared to BPV, i.e. 0.7 vs. 1.4 log. However, BPV was substantially more resistant to heat-trea tment compared to CPV, i.e. 1.3 vs. >3.1 log inactivation after 72 h at 80 degrees C. In a second product, CPV was also slightly more resistant to fre eze-drying than BPV, i.e. 0.2 vs. 1.3 log inactivation. However, heat-treat ment gave essentially similar inactivation for both viruses, i.e. 2.8-3.4 l og after 72 h at 80 degrees C. in conclusion, the resistance of these parvo virus models is dependent both on the type of virus and on the specific pro duct involved. (C) 2000 The International Association for Biologicals.