Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis

Authors
Nandakumar, MP Nandakumar, R Mattiasson, B
Citation
Mp. Nandakumar et al., Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis, BIOTECH LET, 22(18), 2000, pp. 1453-1457
Citations number
15
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
18
Year of publication
2000
Pages
1453 - 1457
Database
ISI
SICI code
0141-5492(200009)22:18<1453:FQOILD>2.0.ZU;2-K
Abstract
A flow injection system for the on-line detection of the intracellular enzy me lactate dehydrogenase (LDH) during fermentation has been developed. The system is comprised of an on-line cell disintegration part, an immobilised dye based expanded bed column for the affinity capture of LDH and a fluorim etric detection unit. The system with a linearity of 0.1-5.4 U LDH ml(-1) w as applied for the detection of intracellular accumulation of LDH during La ctococcus lactis subsp. lactis cultivation.