Effect of anti-P-selectin monoclonal antibody on renal ischemia/reperfusion injury in rats

Objective To explore the effect of anti-P-selectin monoclonal antibody (mAb ) on renal ischemia/ reperfusion (I/R) injury in rats. Methods Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a r enal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochem istry and Nested RT-PCR were used. Results Renal function insufficiency and renal histopathological damage wer e much less in the anti-P-selectin mAb-treated group than the saline-treate d group. Plasma P-selectin levels were lower and renal P-selectin protein a nd mRNA expression were downregulated in the former group. Conclusion Anti-P-selectin mAb might be an efficient approach for the treat ment of renal I/R injury.