Red blood cells from the South American rattlesnake (Crotalus durissus terrificus) regulate volume incompletely following osmotic shrinkage and swelling in vitro
P. Koldkjaer et al., Red blood cells from the South American rattlesnake (Crotalus durissus terrificus) regulate volume incompletely following osmotic shrinkage and swelling in vitro, COMP BIOC A, 127(1), 2000, pp. 49-54
Citations number
22
Categorie Soggetti
Animal Sciences",Physiology
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
The ability of rattlesnake (Crotalus durissus terrificus) red blood cells t
o volume regulate in vitro has been investigated. Blood was drawn through a
catheter inserted in the dorsal aorta and equilibrated to gas mixtures of
different composition. Cells shrunken osmotically by increasing the extrace
llular osmolarity from approximate to 291 mosm l(-1) (n = 3) to approximate
to 632 mosm l(-1) (calculated) only partially regulated their volume back
towards the original volume either at pH 7.51 +/- 0.05 (mean +/- S.D., n =
5) or pH 7.20 +/- 0.06 (mean +/- S.D., n = 3), There was no improvement of
the regulatory volume increase at low haemoglobin oxygen saturation. The li
mited volume restoration was inhibited by separate additions of amiloride (
10(-4) M) or DIDS (10(-4) M) suggesting involvement of the Na+/H+ and Cl-/H
CO3- exchangers. Cells that were swollen osmotically by an approximate to 3
0% dilution of the extracellular medium also exhibited a limited ability to
recover their volume. Therefore, these cells show little ability to volume
regulate when exposed to in vitro conditions that shrink or swell the cell
. (C) 2000 Elsevier Science Inc. All rights reserved.