Vegetation indices derived from reflectance data are related to canopy vari
ables such as aboveground biomass, leaf area index (LAY), and the fraction
of intercepted photosynthetically active radiation (fIPAR). However, under
N stress the relationships between vegetation indices (VI) and these canopy
variables might be confounded due to plant chlorosis. We studied the relat
ionships between reflectance-based VI and canopy variables (aboveground bio
mass, LAP canopy chlorophyll A content [LAI X Chi A], and fIPAR) for a whea
t (Triticum aestivum L.) crop growing under different N supplies. Nitrogen
fertilization promoted significant increases in radiation interception (pla
nt growth) and, to a lesser extent, in radiation use efficiency (RUE). The
VI vs. LAI relationships varied significantly among treatments, rendering t
he VP-based equations unreliable to estimate LAP under contrasting N condit
ions. However, a single relationship emerged when LAI X Chi A was considere
d. Moreover, VP were robust indicators of fIPAR by green canopy components
independently of N treatment and phenology. Aboveground biomass was poorly
correlated with gain yield, whereas cumulative VP simple radio (SR) was a g
ood predictor of grain yield, probably because cumulative SR closely tracke
d the duration and intensity of the canopy photosynthetic capacity.