Utility of restriction fragment analysis for typing herpes simplex virus amplicons following PCR of targets in the DNA polymerase gene

Authors
Podzorski, RP Baker, J Merline, JR Qureshi, R Holsinger, JE
Citation
Rp. Podzorski et al., Utility of restriction fragment analysis for typing herpes simplex virus amplicons following PCR of targets in the DNA polymerase gene, DIAG MICR I, 37(4), 2000, pp. 289-291
Citations number
10
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
ISSN journal
07328893 → ACNP
Volume
37
Issue
4
Year of publication
2000
Pages
289 - 291
Database
ISI
SICI code
0732-8893(200008)37:4<289:UORFAF>2.0.ZU;2-X
Abstract
We compared the utility of restriction endonuclease cleavage to type herpes simplex virus DNA polymerase gene amplicons from two well established PCR primer sets. Amplicons typed using Ava II had a 96% correlation to type det ermined by monoclonal antibody staining, while amplicons typed using Drd I had a 72% correlation to type determined by monoclonal antibody staining. ( C) 2000 Elsevier Science Inc. All rights reserved.