Mjgb. Bugalho et al., Medullary thyroid carcinoma: an accurate pre-operative diagnosis by reverse transcription-PCR, EUR J ENDOC, 143(3), 2000, pp. 335-338
Objective: To study the expression of calcitonin (CT) and thyroglobulin mRN
A in samples of leftover cells in needles used for fine-needle aspiration b
iopsy either from thyroid tumours or cervical lymph nodes.
Patients and methods: Specimens were analysed using reverse transcription-p
olymerase chain reaction; 12 samples from 11 patients were included and mol
ecular diagnosis was compared with cytological or histological diagnosis an
d serum CT measurements.
Results: Transcripts of the CT gene were detected in all six patients with
medullary thyroid carcinoma (MTC) but in none of the other patients.
Conclusions: Present data reinforce this technique as a reliable and altern
ative tool to establish the pre operative diagnosis of MTC, especially when
cytological examination is not conclusive or when cytological information
is not in agreement with clinical data. Furthermore, it may be clinically u
seful to identify those conditions in which increased serum CT in the prese
nce of a thyroid nodule is not due to MTC.