Immobilization of biomolecules in hydrophobically modified carboxymethylcellulose microparticules

This article presents the synthesis of microparticles of carboxymethylcellu lose, and of their derivatives obtained by chemical modifications with hydr ophobic groups (with C-16 or C-16 + C-4 amines). Their physico-chemical pro perties (DS, swelling, etc.) as well as their retention properties were det ermined to test these new supports for immobilization/ separation of biolog ical active substances such as ampicilline, ampicilline//lysozyme mixture a nd other proteins. The hydrophobically modified supports present a high cap acity for adsorption of lysozyme the, the enzymatic activity of lysozyme be ing maintained. (C) 2000 Elsevier Science Ltd. All rights reserved.