Dynamic model of ex vivo granulocytic kinetics to examine the effects of oxygen tension, pH, and interleukin-3

Objective. Evaluating kinetics in hematopoietic cultures is complicated by the distribution of cells over various stages of differentiation and by the presence of cells from different lineages. Thus, an observed response is a n integral response from distributed cell populations. Growth factors and o ther parameters can greatly affect the lineage and maturation stage of the culture outcome. To resolve the kinetics and more clearly define the differ ential effects of O-2 tension (pO(2)), DH, and interleukin-3 (IL-3) on gran ulopoiesis, a mathematical model-based approach was undertaken. Materials and Methods. Granulocytic differentiation is described within a c ontinuous, deterministic framework in which cells develop from primitive gr anulocytic progenitors to mature neutrophils. The model predicts two distri buted populations - quiescent and cycling cells - by incorporating rates of growth, death, differentiation, and transition between quiescence and acti ve cycling. The response of these four model processes to changes in the cu lture environment was examined. Results. Model simulations of experimental data revealed the following: 1) pO(2) effects are exerted only on the growth rate but not maturation times. 2) pH effects between pH 7.25 and 7.4 on growth and differentiation are co upled; however, with increasing oil values, especially at pH 7.6, the death rate for cells in the early stages of differentiation becomes increasingly significant, 3) The absence of IL-3 increases the death rate for primitive cells only minimally but markedly enhances the rate of differentiation thr ough the myeloblast window in the differentiation pathway. The combined eff ects of these environmental factors can be predicted based on changes in th e model parameters derived from the individual effects. Conclusions, Experimental data combined with mathematical modeling can eluc idate the mechanisms underlying the regulation of granulopoiesis by pO(2), pH, and IL-3, The model also can be readily adapted to evaluate the effects of other culture conditions. The increased understanding of experimental r esults gained with this approach can be used to modify culture conditions t o optimize ex vivo production of neutrophil precursors. (C) 2000 Internatio nal Society for Experimental Hematology. Published by Elsevier Science Inc.