Protein G expressed by human group C and G streptococci: Cloning of gene and binding properties

PCR generated fragments of the protein G gene from three GCS and GGS strain s belonging to different G types had been cloned. The resulting PCR product s were cloned into E. coli using expression vector pQE31. The clones, produ cing IgG-binding peptides were selected. Recombinant plasmids carried diffe rent inserts and encoded proteins of different size and with different bind ing properties.