Mechanism for uptake of silica particles by monocytic U937 cells

We examined the mechanism for uptake by monocytic cells of particles found in the atmosphere of some industrial work places. As a model system, irregu lar crystalline silica particles (SPs), sphere-like cryptocrystalline micro silica particles (MPs) and carbon particles (CPs) were exposed to pro-monoc ytic U937 cells. Plasma-treated SP and MP, but not CP, activated the altern ative complement pathway, but bound little C3b. However, all particles adso rbed serum IgG, IgA and IgM unspecifically. Phenotyping of U937 cells for c omplement receptors (CRs) and Fc gamma receptors (Fc gamma Rs) showed that interferon gamma (INF gamma) increased expression of Fc gamma RI, CR3 (CD11 b/CD18) and CR4 (CD11c/CD18) and that phorbol-12-myristate-13-acetate (PMA) increased expression of CR4. Scanning electron microscopy (SEM) demonstrat ed higher phagocytosis of plasma-treated SP than native SP by both PMA- and INF gamma-stimulated, but not unstimulated, cells. MP and CP could not be distinguished from cellular structures. Inhibition experiments in SEM revea led uptake of heparin-plasma - treated SP via Fc gamma RI on INF gamma/-sti mulated U937 cells, but could not exclude possible participation of CR3. Th e results indicate that plasma-treated SPs bind Ig and are internalized by differentiated monocytic cells via Fc gamma RI, which is known to trigger c ellular production of toxic oxygen species that may induce pulmonary inflam mation in vivo.