High-affinity monoclonal antibodies to PED/PEA-15 generated using 5 mu g of DNA

Class-switched, affinity-matured murine monoclonal antibody (MAb) producing cell lines reactive with PED/PEA-15 were generated and isolated in less th an 4 weeks following polynucleotide immunizations using only 5 mu g of DNA in conjunction with the Powderject gene gun. Somatic fusions of peripheral lymph node cells were performed 13 days after initiating delivery of DNA en coding the target antigen. The data presented demonstrates the rapid produc tion, identification, and characterization of class-switched, affinity-matu red MAbs that bind PED/PEA-15, The reported strategy enabled the rapid deve lopment of MAbs that are useful in enzyme-linked immunoadsorbent assay (ELI SA), Western blotting, and immunoprecipitations.