Monoclonal antibodies generated against recombinant ATM support kinase activity

Authors
Alligood, KJ Milla, M Rhodes, N Ellis, B Kilpatrick, KE Lee, A Gilmer, TM Lansing, TJ
Citation
Kj. Alligood et al., Monoclonal antibodies generated against recombinant ATM support kinase activity, HYBRIDOMA, 19(4), 2000, pp. 317-321
Citations number
16
Categorie Soggetti
Immunology
Journal title
HYBRIDOMA
ISSN journal
0272457X → ACNP
Volume
19
Issue
4
Year of publication
2000
Pages
317 - 321
Database
ISI
SICI code
0272-457X(200008)19:4<317:MAGARA>2.0.ZU;2-O
Abstract
We report on the rapid generation of two monoclonal antibodies, ATM A16.35 and ATM. D16.11, that bind to the kinase domain of mutated ataxia telangiec tasia (ATM). These antibodies were generated against E. coli-expressed reco mbinant protein using the RIMMS strategy. We show that ATM A16.35 binds ATM by Western blot analysis, and ATM D16.11 forms immune complexes with nativ e ATM in immunoprecipitations without neutralizing kinase activity.