H. Takahashi et al., Monoclonal antibodies against topoisomerase I suppressed DNA relaxation and HIV-1 cDNA synthesis, HYBRIDOMA, 19(4), 2000, pp. 331-334
Human immunodeficiency virus type 1 (HIV-1) virion is known to carry a numb
er of cellular components including cellular topoisomerase I. Previously, w
e have demonstrated that topoisomerase I enhances HIV-1 cDNA synthesis in r
everse transcription (RT) assays in vitro. In the present study, we have pr
oduced six monoclonal antibodies (MAbs) against human topoisomerase I. The
MAbs suppressed nicking/closing of supercoiled DNA and cDNA synthesis in an
endogenous reverse transcription (ERT) assay using a detergent-disrupted H
IV-1 virion. Thus, the results suggest that topoisomerase I plays an import
ant role in RNA-directed DNA polymerization.