The development of Norway spruce (Picea abies (L.) Karst.) somatic embryos
on a maturation medium was accompanied by changes in nonstructural carbohyd
rate status. During embryo maturation, the content of total soluble sugars
in the embryonal suspensor mass decreased and the partitioning between sucr
ose and hexoses changed considerably in favor of sucrose. Developing somati
c embryos were mainly responsible for these changes. Osmotic stress caused
by the presence of 3.75% polyethylene glycol (PEG) in the maturation medium
(decrease in osmotic potential by 52.5 kPa) resulted in dramatic changes i
n the content of endogenous saccharides. There was a lower total carbohydra
te content in the embryonal suspensor mass grown on the medium containing P
EG in comparison with the untreated control. isolated embryos from later st
ages of embryo development contained mainly sucrose with a small amount (20
%) of fructose and nearly no glucose. A further increase in PEG concentrati
on in the medium (7.5%; decrease in osmotic potential by 112.5 kPa compared
to the maturation medium) led to a large increase in the total endogenous
sugar content. This increase in sugars was a result of the enhanced content
of sucrose, fructose, and glucose. The increased glucose content was in co
ntrast to embryos grown on the medium with lower or no PEG content.