MOLECULAR-CLONING OF THE DOG BETA-1 AND BETA-2-ADRENERGIC RECEPTORS

We report the isolation of the genes encoding the beta 1 and beta 2 ad renergic receptors from dog genomic DNA. Sequence analysis of both gen es revealed intronless open reading frames of 473 and 415 amino acid r esidues, receptively. Heterologous expression of both receptors in CHO cells indicated that both receptors are functionally similar to the h uman homologs. Comparing the dog beta 1 and beta 2 adrenergic receptor s, the beta 1 receptor appears to bind to G proteins more tightly than the beta 2 receptor. Heterologously expressed receptors provide a con venient system for evaluating novel receptor agonists and antagonists.