Leaf flavonoids were quantitatively determined in 68 representative or econ
omically important Citrus species, cultivars, and near-Citrus relatives. Co
ntents of 23 flavonoids including 6 polymethoxylated flavones were analyzed
by means of reversed phase HPLC analysis. Principal component analysis rev
ealed that the 7 associations according to Tanaka's classification were obs
erved, but some do overlap each other. Group VII species could be divided i
nto two different subgroups, namely, the first-10-species class and the las
t-19-species class according to Tanaka's classification numbers.