Polymorphism in indigenous poultry germplasm detected through randomly amplified polymorphic DNA

This study involved the use of randomly amplified polymorphic DNA (RAPD) ma rkers in detecting the polymorphism between two important native chicken br eeds viz. Aseel and Kadaknath and to estimate the genetic variability betwe en them. Out of 30 primers screened, 24 could generate distinct and reprodu cible RAPD patterns and only 7 of these, detected the polymorphism between these two native breeds. Thirteen of the 63 amplicons generated from the 7 selected primers, were found to be polymorphic. The Aseel population (WS = 0.91 to 0.96) showed higher within breed genetic similarity in comparison t o the Kadaknath (WS = 0.81 to 0.82) from either measure of genetic similari ty. Both the breeds showed moderate genetic distances (0.108) between them. Some selected primers could detect polymorphism between two varities of As eel i.e. Peela and Kala.