Adsorption of peroxidase on titanium surfaces: A pilot study

The present study demonstrates the in vitro and in vivo adsorption of perox idase onto titanium surfaces. Titanium foils (mean +/- SEM: 365 +/- 2 mm(2) , n = 114) were incubated during 30 min with lactoperoxidase (4 mg in 5 mL 100 mM phosphate buffer pH 7). After 15 washings by H2O, titanium foils wer e incubated with o-phenylenediamine (6 mg/mL) and H2O2 (7 mM) during 30 min . The reaction was then stopped by the addition of HCl 1M and the absorbanc e of the liquid phase was read on a spectrophotometer at 492 nm. In vitro a dsorbed lactoperoxidase onto titanium surfaces was 0.70 +/- 0.05 ng/mm(2) ( mean +/- SEM, n = 30). X-ray photoelectron spectroscopy confirmed the incor poration of protein nitrogen onto titanium surfaces: the nitrogen atomic pe rcentage increased from 0.9 +/- 0.3 to 12.7 +/- 0.2% (n = 3) and from 3.7 /- 0.1 to 14.4 +/- 0.4% (n = 5) when titanium foils were incubated in the l actoperoxidase solution during 30 min and 24 h respectively. In vivo, oral peroxidases adsorbed on titanium healing abutments from 0.01 to 0.58 ng/mm( 2) (n = 19) after 2 weeks in the oral environment. (C) 2000 John Wiley & So ns, Inc.