Human serum albumin chromatography by Cibacron Blue F3GA-derived microporous polyamide hollow-fiber affinity membranes

An affinity dye ligand, Cibacron Blue F3GA was covalently attached onto com mercially available microporous polyamide hollow-fibre membranes for human serum albumin (HSA) adsorption from both aqueous solutions and human plasma . Different amounts of Cibacron Blue F3GA were incorporated on the polyamid e hollow-fibres by changing the dye attachment conditions, i.e. initial dye concentration, addition of sodium carbonate and sodium chloride. The maxim um amount of Cibacron Blue F3GA attachment was obtained at 42.5 mu mol g(-1 ) when the hollow-fibres were treated with 3 M HCl for 30 min before perfor ming the dye attachment. HSA adsorption onto unmodified and Cibacron Blue F 3GA-derived polyamide hollow-fibre membranes was investigated batchwise. Th e non-specific adsorption of HSA was very low (6.0 mg g(-1) hollow-fibre). Cibacron Blue F3GA attachment onto the hollow-fibres significantly increase d the HSA adsorption (147 mg g(-1) hollow-fibre). The maximum HSA adsorptio n was observed at pH 5.0. Higher HSA adsorption was observed from human pla sma (230 mg HSA g(-1) hollow-fibre). Desorption of HSA from Cibacron Blue F 3GA derived hollow-fibres was obtained using 0.1 M Tris-HCl buffer containi ng 0.5 M NaSCN or 1.0 M NaCl. High desorption ratios (up to 98% of the adso rbed HSA) were observed. It was possible to reuse Cibacron Blue F3GA derive d polyamide hollow-fibre without significant decreases in the adsorption ca pacities. (C) 2000 Elsevier Science B.V. All rights reserved.