Solid-phase extraction and liquid chromatographic quantitation of quinfamide in biological samples

This paper describes a high-performance liquid chromatographic method for t he assay of quinfamide and its main metabolite, 1-(dichloroacetyl)-1,2,3,4, -tetrahydro-6-quinolinol, in plasma, urine and feces. It requires 1 ml of b iological fluid, an extraction using Sep-Pack cartridges and acetonitrile f or drug elution. Analysis was performed on a CN column (5 mu m) using water -acetonitrile-methanol (40:50:10) as a mobile phase at 269 nm. Results show ed that the assay was linear in the range between 0.08 and 2.0 mu g/ml. The limit of quantitation was 0.08 mu g/ml. Maximum assay coefficient of varia tion was 14%. Recovery obtained in plasma, urine and feces ranged from 82% to 98%. (C) 2000 Elsevier Science B.V. All rights reserved.