Determination of gestrinone in human serum by liquid chromatography-electrospray tandem mass spectrometry

A rapid, sensitive and specific high-performance liquid chromatography-elec trospray tandem mass spectrometric method has been developed for the determ ination of gestrinone (R 2323) in human serum using mifepristone (RU 486) a s an internal standard. R 2323 was extracted from human serum by an ether e xtraction procedure. Multiple reaction monitoring was used to detect R 2323 and RU 486. The calibration curve was linear over the range of 3.5-177 ng/ ml (r(2)greater than or equal to 0.99) with the limitation of detection of 0.8 ng/ml. The intra-day precision and accuracy, expressed as C.V. and RE, ranged from 2.3-13.7 to -4.8-3.0%. The inter-day precision and accuracy ran ged from 5.5-14.8 to -6.7-3.1%. The mean recovery was 91.0% for R 2323, and 90.6% for the internal standard. The method was successfully applied to th e pharmacokinetic study of R 2323. (C) 2000 Elsevier Science B.V. All right s reserved.