Simultaneous determination of remacemide hydrochloride and desglycinylremacemide (AR-R12495XX) in brain tissue by high-performance liquid chromatography
S. Santangeli et al., Simultaneous determination of remacemide hydrochloride and desglycinylremacemide (AR-R12495XX) in brain tissue by high-performance liquid chromatography, J CHROMAT B, 746(2), 2000, pp. 325-329
Remacemide hydrochloride, a novel anticonvulsant agent, and its major activ
e metabolite, desglycinylremacemide, were measured simultaneously in brain
tissue by high-performance liquid chromatography with UV detection. Intra-
and inter-assay variations for remacemide (1, 5, 10 mu g/ml) were 5.1, 10.5
and 3.1% and 3.1, 4.0 and 1.3%, respectively. Intra- and inter-assay varia
tions for desglycinylremacemide (1, 5, 10 mu g/ml) were 4.2, 3.8 and 8.4% a
nd 7.9, 8.8 and 3.1%, respectively. Limits of detection and quantification
for both analytes were 4 and 31 ng/ml, respectively, with recovery consiste
ntly greater than or equal to 85%. This reliable assay has applications in
the pre-clinical neuropharmacokinetic and neuropharmacodynamic investigatio
n of remacemide hydrochloride. (C) 2000 Elsevier Science B.V. All rights re
served.