Solution-phase synthesis of a 1,5-dialkylamino-2,4-dinitrobenzene library and the identification of novel antibacterial compounds from this library

In this report we demonstrate that a 1,5-dialkylamino-2,4-dinitrobenzene sm all molecule library can be generated by a highly efficient solution-phase synthesis method. From this 2485-member library, a series of novel compound s with antibacterial activity were isolated. The significance of this repor t is that the synthetic scheme is extremely simple, with minimal number of liquid handling steps, and the solvents and reagents left in the final libr ary preparation are fully compatible with cell-based assays.