Soil management is one of the most important factors influencing the struct
ure of soils. The interaction of management (including tillage and crop rot
ation history) with soil biochemistry, soil aggregation, and soil humus com
position was determined in a native prairie and a producer field situation
in 1997. A comparison of a native prairie and an adjacent conventional corn
(Zea mays L.)-soybean [Glycine max (L.) Merr.] rotation on the same soil t
ype found that the Webster soil (fine-loamy, mixed, superactive, mesic Typi
c Endoaquoll) after soybean (Cs plant) was lower in monosaccharide content
and protein content as determined by ion chromatography, and lower in pheno
lic acid content than the Webster soil after corn (C-4 plant) or in native
prairie as determined by gas chromatography. A wet, nested sieve aggregate
stability measurement determined that the prairie soil had a higher mean ag
gregate size (1.85 mm) when compared with the soil in the presence of decom
posing corn (1.0 mm) or soybean (0.34 mm) residues. Mean aggregate size was
found to be correlated with soil monosaccharide content (r = 0.75), total
soil protein content (r = 0.995***), total soil phenolic acid content (r =
0.997***), and alkaline extractable humic substance content (r = 0.98**). A
lkaline extractable humic substances were correlated with the phenolic acid
content of the humic substances (r = 0.996***). The results suggest that t
he decrease in soil stability after soybean growth was due to a decrease in
the content of soil humic substances caused by the substantially lower phe
nolic adds content (humic acid precursors) in the soybean residue.