Role of pulmonary surfactant protein D in innate defense against Candida albicans

Pulmonary surfactant protein D (SP-D), which is a member of the collectin f amily, is implicated in pulmonary defense against pathogens. To determine w hether SP-D is involved in first-line immunity against Candida albicans, an important respiratory fungus, the interaction of SP-D with C. albicans was studied. SP-D was found to bind C. albicans, resulting in agglutination of the microorganisms. Binding was calcium dependent and was inhibited by com peting sugars maltose or mannose, Incubation of C. albicans with SP-D resul ted in profound fungal growth inhibition and decreased hyphal outgrowth. Fu rthermore, it was found that SP-D inhibited phagocytosis of C. albicans by alveolar macrophages. These data suggest that the lung collectin SP-D has a n important role in first-line defense against C. albicans in the lung, by agglutinating C. albicans and limiting their growth, without the need for m acrophage activation.