Pseudonomas cepacia Lipase supported on ceramic particles (lipase PS-C) and
diatomite (lipase PS-D) catalyzed the acylation of aldoximes and ketoximes
. Aliphatic oximes reacted faster than aromatic oximes and polar solvents e
nhanced the reaction rate. For lipase PS-C, THF was a superior solvent, whi
le for lipase PS-D 1,4-dioxane was the ideal solvent. The amount of enzyme
required to catalyze this reaction was optimized. It was found that for Lip
ase PS-C or lipase PS-D, 50 mg was the optimum amount to catalyze 1.0 mmol
of substrate. (C) 2000 Elsevier Science B.V. All rights reserved.