CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin

We report on the comparative analysis of the conformation-sensitive bands o f free enzyme (chymotrypsin), liganded enzyme (chymotrypsin anthranilate) a nd enzyme complex with 18-crown-6, The studies were carried out by Raman sc attering spectroscopy and polarization-sensitive coherent anti-Stokes Raman scattering spectroscopy (CARS). The bands of tyrosine and tryptophan resid ues, disulfide bridges and amide I and amide III were analyzed. It is concl uded that the conformation of the enzyme changes much more in the case of l iganding than in the case of the formation of a complex with the crown ethe r. Copyright (C) 2000 John Wiley & Sons, Ltd.