O. Reany et al., A model system using modulation of lanthanide luminescence to signal Zn2+ in competitive aqueous media, J CHEM S P2, (9), 2000, pp. 1819-1831
Citations number
41
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
Two pentadentate tribasic ligand systems containing aniline or benzylamine
nitrogens covalently linked to a proximate kinetically stable Eu or Tb comp
lex are described. The affinity of these complexes and their non-conjugated
analogues for Zn2+, Ca2+ and Mg2+ ions has been measured at ambient pH in
a high salt background. Apparent binding constants for the parent ligands (
L-1: Zn2+ log beta(ML) 5.04, Ca2+ 3.91, Mg2+ 2.1, L-3: Zn2+ 5.93, Ca2+ 5.00
, Mg2+ 3.60) were slightly lowered in the aniline-based terbium conjugate [
TbL4], and were the same for the benzylamine-based conjugate [LnL(2)], exce
pt for zinc binding for which a slightly enhanced affinity was observed. Ch
anges in the form of ligand absorption and emission spectra and in the inte
nsity of delayed lanthanide luminescence characterised metal ion binding. W
ith [LnL(2)], a 42 and 26% increase in emission at 700 nm (Eu) and 545 nm (
Tb) accompanied zinc binding in a simulated extracellular background, with
an apparent dissociation constant of 0.6 mu M (295 K).