Reaction of superoxide with phenoxyl-type radicals

Radiolytically generated phenoxyl radicals derived from the structurally si milar phenols cresol, tyrosine, tyramine and tyrosol were reacted with O-2( .-) [k=(1-4.5)x10(9) dm(3) mol(-1) s(-1), by pulse radiolysis] and the cons umption of the phenol determined. Although the reduction potentials of thes e phenoxyl radicals are very close, ranging between +0.64 and +0.68 V, the yields of phenol consumption vary considerably [between 8% (tyrosol) and 90 % (tyrosine)]. This indicates that electron transfer with restitution of th e phenol does not necessarily occur to a major extent, although this reacti on is thermodynamically favoured (reduction potential of O-2(.-), -0.33 V). The reactivity of phenoxyl radicals from phenols that are structurally dif ferent from the above has also been studied. In the case of the reaction of O-2(.-) with 2,4,6-trimethylphenoxyl, hydroperoxides are formed which reve rt to the phenol by eliminating dioxygen (perhaps in the singlet state) in a slow reaction on a time-scale of many minutes. The rate of this reaction increases with increasing pH and increasing temperature. From these data it is calculated that the hydroperoxides from 2,4,6-trimethylphenol have a pK (a) of 11.3 and that the reaction requires an activation energy of 105 kJ m ol(-1) (frequency factor, 3.5x10(14) s(-1)). In competition with the elimin ation of dioxygen, depending on the presence of suitable substituents, such hydroperoxides can undergo other reactions. In the case of tyrosine, there is a cyclization reaction followed by the elimination of hydrogen peroxide . Phenoxyl radicals that carry hydrogen in the ortho or para-position (e.g. such as those derived from 4-methylphenol, 2,6-dimethylphenol or 2-methoxy -4-methylphenol) give rise to hydroperoxides which can eliminate water to p roduce the corresponding quinones. These subsequently suffer stepwise reduc tion by O-2(.-) to the corresponding catechols or hydroquinones.