Special modes of development of Chelidonium majus L. alkaloids in systems of the type polar adsorbent-multicomponent mobile phase by TLC

Special modes of development such as unidimensional multiple development (U MD), stepwise gradient development, and manual operation of automated multi ple development (AMD, reversed gradient) have been used for the microprepar ative separation of tertiary and quaternary alkaloid mixtures from Chelidon ium majus L. roots. When the separations were compared with analytical-scal e separations of identical mixtures it was found that the quaternary or ter tiary alkaloids are well separated by use of chromatographic systems of the type silica - multicomponent mobile phase and alumina (basic) - methanol dichloromethane mixture. The best results from zonal micropreparative sepa ration of the alkaloid mixtures were obtained by use of unidimensional trip le development and stepwise gradient elution.