Elimination of aflatoxin M-1 with the milk of lactating cows after simultaneous feeding of adsorptive additives and experiments with aflatoxin B-1 - in vivo and in vitro

To verify the advertising of the producers for bentonites/montmorillonites and feed yeast extracts as regards efficient adsorption of aflatoxin and th us reduced bioavailability, products actually offered on the market (5 clay minerals, 2 yeast extracts) were analyzed on their adsorption characterist ic of aflatoxin B-1 in the lactating cow and, if applicable, on the reduced elimination of aflatoxin M-1 with milk. Pursuant to the feedstuff regulation as regards the directives for feed sup plements, or following indications of the producers two lactating cows were supplemented AFB(1) (50 mu g/day) and simultaneously a sorbent in a suspen sion (40-400g/day) at each of the 4 daily feedings. Afterwards, the concent ration of AFM, in milk was measured via ELISA. The mineral products showed moderate effects leading on average to a reduce d elimination of approx. 14 %. Although the differences depending on the in dividual animal led to a decrease of 32% they also stimulated the AFM, elim ination by 36 %. The esterified glucomanoses of yeast had nearly no effect on the aflatoxin content of milk. The in-vitro assays concerning adsorption featured similar results. Bentoni tes demonstrated a relatively high adsorption of aflatoxin. B-1 from propio nic acid. Adding epoxidases to the adsorbent decreased the adsorption capac ity whereas the adsorption of clinoptilolith was more or less low, probably due to the molecular sieve effect. For all the analyzed systems, no satura tion of the adsorbents was registered.