Transgenic resistance to the nematode Rotylenchulus reniformis conferred by Arabidopsis thaliana plants expressing proteinase inhibitors

The level of resistance to the reniform nematode Rotylenchulus reniformis c onferred on Arabidopsis thaliana by transgenic expression of proteinase inh ibitors (PI) was studied over 40 days of infection. A cysteine PI differing from Oc-I expressed in rice seed by one amino acid (Oc-I Delta D86) had a considerable effect. It reduced parasite density and fecundity to 65 +/- 4% and 31 +/- 8% respectively, relative to values for untransformed plants wh en expressed at ca. 0.4% total soluble protein. The overall reproductive su ccess was only 19 +/- 5% relative to the controls. Similar expression level s of cowpea trypsin inhibitor, a serine PI, reduced the density of the fema les on the plants to 77 +/- 3% of control values but did not reduce their f ecundity. There was no evidence for additive effects when both PIs were exp ressed as a fusion protein using a peptide linker. Both PIs in the fusion P I protein are known to be functional from our previous work. When developin g on A. thaliana plants expressing the transgenic proteins the intact PI fu sion protein of 22 kDa was detected in nematodes by western blot analysis. Similarly, R. reniformis females were observed to have ingested Green Fluor escent Protein (28 kDa). Demonstrating the ingestion of the fusion protein, which has been associated with additive effects of the two PIs against oth er nematodes, supports the observation that serine PIs are of less value th an cysteine PIs for R. reniformis control. The effect of Oc-I Delta D86 on R. reniformis was dependent on the level of expression of different A. thal iana lines in the range of 0.1-0.4% total soluble protein indicating that t he upper level of expression is required for an effective defence. Virtual northern analysis with cysteine and serine proteinase gene probes from Hete rodera glycines showed high transcript abundance of a serine proteinase in both pre-parasitic and parasitic stages whereas expression of the cysteine proteinase was more abundant in the feeding adult female. This study provid es further evidence that cystatins represent a valuable source of control a gainst a range of economic nematodes including Globodera, Heterodera and Me loidogyne and now including R. reniformis. Preliminary screening in A. thal iana provides a convenient guide for selection of transgenes against nemato de pathogens of crops such as pineapple that have a prolonged regeneration period.