Cuparene-type sesquiterpenes were isolated from a culture broth of Flammuli
na velutipes (Curt.:Fr.) Sing. Using spectroscopic methods (HR-MS,H-1 and C
-13 NMR, and 2D NMR, spectroscopy), their structures were determined to be
2,3,4,5-tetrahydro-2,7-dihydroxy-5,8,10,10-tetramethyl-2,5-methano-1-benzox
epin and 5-methyl-2-(3-oxo-1,2,2-trimethylcyclopentyl)benzoquinone. Both sh
owed antimicrobial activity against Cladosporium herbarum and Bacillus subt
ilis. (C) 2000 Elsevier Science Ltd. All rights reserved.