Quantification of Ca2+/H+ antiporter VCAX1p in vacuolar membranes and its absence in roots of mung bean

Authors
Ueoka-Nakanishi, H Maeshima, M
Citation
H. Ueoka-nakanishi et M. Maeshima, Quantification of Ca2+/H+ antiporter VCAX1p in vacuolar membranes and its absence in roots of mung bean, PLANT CEL P, 41(9), 2000, pp. 1067-1071
Citations number
23
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT AND CELL PHYSIOLOGY
ISSN journal
00320781 → ACNP
Volume
41
Issue
9
Year of publication
2000
Pages
1067 - 1071
Database
ISI
SICI code
0032-0781(200009)41:9<1067:QOCAVI>2.0.ZU;2-E
Abstract
Vacuolar Ca2+/H+ antiporter VCAX1p, which contributes to the Ca2+ accumulat ion into vacuoles, was quantified by immunochemistry. The antiporter conten t in vacuolar membranes was 0.14 and 1.1 mu g mg(-1) of membrane protein fo r hypocotyls and epicotyls, respectively. The calculated turnover number wa s 120 s(-1). Roots lacked the antiporter protein and the transcript.