Ic. Madakadze et al., Effect of preplant seed conditioning treatment on the germination of switchgrass (Panicum virgatum L.), SEED SCI T, 28(2), 2000, pp. 403-411
Switchgrass (Panicum virgatum. L.) is being developed and evaluated as summ
er forage and for biomass production in eastern Canada. Uneven germination
and slow seedling growth in spring are some of the factors limiting its cul
tivation. A study was conducted to reduce seed dormancy and to improve germ
ination at suboptimal temperatures in switchgrass cultivars Cave-in-Rock (C
IR), Dakota (DK) and New Jersey 50 (NJ). Seeds of these cultivars were cond
itioned either with 0.2% potassium nitrate (KNO3) or 1 mM gibberellic acid
or osmoconditioned with polyethylene glycol 8000 (PEG) solution with or wit
hout 0.2% KNO3 or 1 mM gibberellic acid (GA(3)); or matriconditioned with M
icro-Gel E (MC) with either water, 0.2% KNO3 or 1 mM GA(3). The seeds were
conditioned at 8 or 16 degrees C for 4 days and then germinated at 8, 16 or
24 degrees C. Conditioning treatment and temperature influenced germinatio
n. For CIR seeds germinated at 8 degrees C, conditioning with PEG at 8 degr
ees C increased germination from 0 (control) to 22%. All the conditioning t
reatments germinated earlier and the proportion of seeds that eventually ge
rminated was higher than for the unconditioned control. In the cultivar NJ
conditioning at 16 degrees C with water or 1 mM GA(3) increased germination
to 12 and 17% respectively (0 for the control) when seeds were germinated
at 80C. When germinated at 16 degrees C treatments containing 1 mM GA, germ
inated earlier. Osmoconditioning in PEG with 1 mM CA, had the highest final
germination at 56% (37% for control). Cultivar DK did not significantly re
spond to the various conditioning treatments. Overall, both osmoconditionin
g and matriconditioning hastened germination and total germination in switc
hgrass cultivars CIR and NJ.