Varietal discrimination of tomato (Lycopersicon esculentum L.) by ultrathin-layer isoelectric focusing of seed protein

Authors
Wang, XF Knoblauch, R Leist, N
Citation
Xf. Wang et al., Varietal discrimination of tomato (Lycopersicon esculentum L.) by ultrathin-layer isoelectric focusing of seed protein, SEED SCI T, 28(2), 2000, pp. 521-526
Citations number
10
Categorie Soggetti
Plant Sciences
Journal title
SEED SCIENCE AND TECHNOLOGY
ISSN journal
02510952 → ACNP
Volume
28
Issue
2
Year of publication
2000
Pages
521 - 526
Database
ISI
SICI code
0251-0952(2000)28:2<521:VDOT(E>2.0.ZU;2-W
Abstract
Ultrathin-layer isoelectric focusing (UTLIEF) of seed proteins soluble in 4 mmol l(-1) NaCl was used to discriminate tomato varieties. Eight polymorph ic protein bands were found on the gel with a pH range of 4-8, of which six bands were reported for the first time. About 87% of pairwise comparisons of the 11 varieties tested showed at least one different protein band. Almo st all varieties were found to be not homozygous genetically.