Short communication - Inoculum density-dependent restriction of nodulationin the soybean-bradyrhizobium japonicum symbiosis

Bradyrhizobium japonicum USDA 110 is restricted for nodulation by Glycine m ax PI 417566 at relatively high inoculum levels (10(8)-10(9) cells). Nodula tion restriction conditioned by PI 417566 was suppressed at inoculation lev els of 10(4)-10(6) cells, although nodulation was delayed relative to that seen on a permissive soybean genotype. Over the range of concentrations tes ted (10(4)-10(9) cells) on soybean cv. Kasota, there was no influence of in oculum concentration on nodulation by USDA 110 or by D4.2-5, a Tn5-induced, nodulation-competent mutant of USDA 110. The few nodules produced by USDA 110 on PI 417566 contained normal levels of nodulin-23, nodulin-26, cdc-2 g ene, and LB23. Phenylalanine ammonia lyase mRNA levels were about 15% great er, 2 days post inoculation, when PI 417566 was inoculated with USDA 110 th an was seen when strain D4.2-5 was the inoculum. There were no differences seen in the expression of chalcone synthase in nodulation-permissive or -re strictive plant genotypes inoculated with USDA 110 or D4.2-5. These results suggest that inoculum size and host defense-related responses may influenc e host-controlled restriction of nodulation in the B. japonicum soybean sym biosis.