A mild method for fluorescein labeling of base-sensitive oligonucleotides on solid support

Authors
Bologna, JC Imbach, JL Morvan, F
Citation
Jc. Bologna et al., A mild method for fluorescein labeling of base-sensitive oligonucleotides on solid support, TETRAHEDR L, 41(38), 2000, pp. 7317-7321
Citations number
17
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
41
Issue
38
Year of publication
2000
Pages
7317 - 7321
Database
ISI
SICI code
0040-4039(20000916)41:38<7317:AMMFFL>2.0.ZU;2-J
Abstract
A simple and mild method is described to covalently label base-sensitive su pported oligonucleotides, such as prooligonucleotides, with unprotected car boxyfluorescein as a highly reactive dimethylaminopyridinium ester generate d in situ. (C) 2000 Elsevier Science Ltd. All rights reserved.