Identification of cyprinol and cyprinol sulfate from grass carp bile and their toxic effects in rats

To elucidate the responsible toxic components of grass carp bile, the bile salt 5 alpha-cyprinol sulfate and its desalted form 5 alpha-cyprinol from g rass carp bile were purified and identified by analyses of infrared spectru m, H-1-, C-13-nuclear magnetic resonance spectra and mass spectrum. The tox icity of grass carp bile powder, butanol extract of grass carp bile powder, 5 alpha-cyprinol and 5 alpha-cyprinol sulfate in rats were further determi ned. The kidney and liver functions were significantly affected by grass ca rp bile powder, butanol extract and 5 alpha-cyprinol sulfate. However, 5 al pha-cyprinol also significantly affected the kidney function, but the toxic effect was less. (C) 2000 Elsevier Science Ltd. All rights reserved.