Little is known about the role of interleukin-10 (IL-10), an antiinflammato
ry cytokine, in blood vessels. We used IL-10-deficient mice (IL-10 -/-) to
examine the hypothesis that IL-10 protects endothelial function after lipop
olysaccharide (LPS) treatment. The responses of carotid arteries were studi
ed in vitro 6 h after injection of a relatively low dose of LPS (10 mu g ip
). In IL-10 -/- mice, the maximum relaxation to ACh (3 mu M) was 56 +/- 6%
(means +/- SE) after LPS injection and 84 +/- 4% after vehicle injection (P
< 0.05). Thus endothelium-dependent relaxation was impaired in carotid arte
ries from IL-10 -/- mice after LPS injection. In contrast, this dose of LPS
did not alter relaxation to ACh in vessels from wild-type (IL-10 +/+) mice
. Relaxation to nitroprusside and papaverine was similar in arteries from b
oth IL-10 -/- and IL-10 +/+ mice after vehicle or LPS injection. Because in
flammation is associated with increased levels of reactive oxygen species,
we also tested the hypothesis that superoxide contributes to the impairment
of endothelial function by LPS in the absence of IL-10. Results using conf
ocal microscopy and hydroethidine indicated that levels of superoxide are e
levated in carotid arteries from IL-10 -/- mice compared with IL-10 +/+ mic
e after LPS injection. The impaired relaxation of arteries from IL-10 -/- m
ice after LPS injection was restored to normal by polyethylene glycol-suspe
nded superoxide dismutase (50 U/ml) or allopurinol (1 mM), an inhibitor of
xanthine oxidase. These data provide direct evidence that IL-10 protects en
dothelial function after an acute inflammatory stimulus by limiting local i
ncreases in superoxide. The source of superoxide in this model may be xanth
ine oxidase.